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There was one large discrepancy between the DNA and RNA data. Relatively large amounts of *Ae*. *albopictus* RNA was present in the sand fly pools, but no genomic DNA. This might be due to the fact that during RNA isolation, the nucleic acids from the inactive stages of the parasite (eggs and larvae) would be separated and consequently enriched, whereas genomic DNA would be largely depleted. In these type of experiments, the extraction of RNA from whole sand fly pools should be performed to ensure the correct data. These results provide important insights into the mechanisms used by *Ae*. *albopictus* to resist various insecticides in the laboratory. Also, they highlight the importance of including biological replicates in field testing experiments. Field observations have shown that *Ae*. *albopictus* can maintain low levels of resistance to pyrethroids \[[@pone.0199436.ref017]\], and weak resistance to DDT \[[@pone.0199436.ref018]\] and carbamates \[[@pone.0199436.ref015]\], in temperate European populations of *Ae*. *albopictus*. Our findings are consistent with these field studies, showing that *Ae*. *albopictus* will accumulate mutations after a single selection with the pyrethroids deltamethrin and lambda-cyhalothrin, and that there is no correlation between the resistance to pyrethroids and DDT. When considering the effective dosage, it is important to note that many studies have focused on adult females of *Ae*. *albopictus* \[[@pone.0199436.ref015], [ 91bb86ccfa EPageCreator License Key ePageCreator is a handy utility for converting PDF, SWF and image files to HTML, EXE, APP, ePub and MOBI formats. 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